Anti-B7-H3 (CD276) Antibody, clone MJ8

Description

• General description: B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
• Specificity: This antibody recognizes the extracellular domain of B7-H3 (CD276).
• Immunogen: Epitope: Extracellular Domain
• Application: Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.
• Quality: Evaluated by Western Blot in human placenta tissue lysate.Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.
• Target description: ~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Analysis Note: ControlHuman placenta tissue lysate
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.