Monoclonal Anti-Fas

Description

• General description: Monoclonal Anti-Human Fas (CD95/Apo-1) (mouse IgG1 isotype) is derived from the DX2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from C3H mice immunized with murine L cells transfected by a human Fas/CD95 cDNA. CD95/Fas/Apo-1 exhibits strong homologies with the extracellular domain of receptors belonging to the tumor necrosis factor (TNF) receptor family, which includes TNF receptor types 1 and 2 (TNFR1/2), the low affinity nerve growth factor receptor, and lymphocyte receptors such as CD27, CD30, CD40, and OX40. Fas is an integral membrane protein, with strong homology to TNF-α and -β , has been identified as Fas ligand.
• Specificity: Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.
• Immunogen: murine L cells transfected with a human Fas/CD95 cDNA.
• Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)
• Biochem/physiol Actions: Fas is expressed in a number of lymphoma cell lines, on Epstein-Barr virus-transformed B lymphoblasts and on a proportion of activated B and T cells. Fas is also detected in soluble form and this form of the protein is thought to play a role in regulating certain aspects of immune system function. Elevated levels of soluble Fas is observed in leukemia and systemic lupus erythematosus. Therefore, altered levels of secreted Fas protein is likely to be involved in the abnormal growth regulation of lymphoid cells.
• Physical form: Solution from a bioreactor culture supernatant in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.