Anti-FcγRIIb/c Antibody, clone 4F5

Description

• General description: FcγRI/CD64, FcγRII/CD32, FcγRIII/CD16, and FcγRIV/CD16-2 represent the four known classes of IgG Fc receptors (FcγRs). FcγRII, FcγRIII, and FcγRIV are low-affinity receptors for monomeric IgG, whereas FcγRI is the only high-affinity FcγR. FcγRs play important roles in inflammatory cell activation, clearance, presentation of Ag, and maintenance of IgG homeostasis. In addition to binding immune complex (IC), FcγRs have been shown to bind non-IgG ligands. For example, FcγRII is shown to bind oxLDL, while FcγRIII binding to an E. coli component is reported to negatively regulate the function of macrophage receptor with collagenous structure (MARCO). FcyRII activation is also reported to transduce a negative regulatory signal against CD38 crosslinking-induced proliferation of resting mature B cells. Low affinity immunoglobulin gamma Fc region receptor II-a/b/c (IgG Fc receptor II-a/-b/-c, CDw32, Fc-gamma RII-a/-b/-c, Fc-gamma-RIIa/b/c, FcRII-a/-b/-c, CD32) are encoded by the FCGR2A/B/C (CD32, FCG2, IGFR2) genes in human (UniProt P12318, P31994, P31995; Gene ID 2212, 2213, 9103). The FcγRIIs encoded by the three genes are highly conserved in their extracellular portion, with significant variation in the transmembrane and intracellular regions. All three types of transcripts alternative splicing to generate additional isoforms.
• Specificity: Clone 4F5 recognizes human FcγRIIb and FcγRIIc, but not any of the two polymorphic forms of human FcγRIIa with either Arg131 or His131 (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175; Su, K., et al. (2007). J. Immunol. 178(5):3272-3280). Expected to bind all spliced isoforms of human FCGR2B and FCGR2C reported by UniProt (P31994 & P31995).
• Immunogen: Epitope: Extracellular domain.
• Application: Anti-FcγRIIb/c Antibody, clone 4F5 is an antibody against FcγRIIb/c for use in Immunohistochemistry (Paraffin), Flow Cytometry, Immunoprecipitation, Western Blotting, Immunocytochemistry.
• Quality: Evalulated by Immunohistochemistry in human pancreas tissue.Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected FcγRIIb/c immunoreactivity in Islets of Langerhans in human pancreas tissue sections.
• Target description: 29.49 kDa (FcγRIIB1), 27.39 kDa (FcγRIIB2), 29.22 kDa (FcγRIIB3), 31.03 kDa (FcγRIIC1), 25.69 kDa (FcγRIIC2), 24.82 kDa (FcγRIIC3), 21.35 kDa (FcγRIIC4) calculated.
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Legal Information: ALEXA FLUOR is a trademark of Life Technologies
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.