Anti-USP7 antibody, Rat monoclonal

Description

• General description: Monoclonal Anti-USP7 (rat IgG2a isotype) is derived from the hybridoma USP 3D8 produced by the fusion of mouse myeloma cells (P3X63 AG 8.653) and splenocytes from rat immunized with a peptide.
• Specificity: Monoclonal Anti-USP7 recognizes human and rat USP7.
• Application: Monoclonal Anti-USP7 antibody produced in rat may be used in:immunoblottingimmunoprecipitationimmunocytochemistry
• Biochem/physiol Actions: Overexpression of the USP7 gene results in p53-mediated apoptosis. Ablation of the USP7 gene expression results in p53 accumulation as opposed to the expected destabilization of p53. This effect has been traced to the ability of USP7 to stabilize mouse double minute 2 homolog (MDM2), a ubiquitin ligase that promotes the degradation of p53. Therefore, USP7 appears to play multiple roles in regulating the p53-MDM2 pathway and in maintaining steady-state levels of p53 in the cell. USP7 is considered for treating hematopoietic tumors due to its role in p53 regulation.
• Target description: USP7 is ubiquitin specific peptidase 7 (herpes virus-associated). It is associated with induction of apoptosis, interspecies interaction between organisms, protein deubiquitination, regulation of protein stability, and ubiquitin-dependent protein cataboli
• Physical form: Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
• Storage and Stability: Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.