Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit

Description

• General description: Cytosolic phospholipase A2, group IVa, (cPLA2, also known as cPLA2a, PLA2G4A) is a member of the PLA2 superfamily that catalyses the cleavage of fatty acids from the sn-2 position of phospholipids. PLA2 isoenzymes vary in their cellular localizations, Ca2+ sensitivities and substrate specificities. cPLA2 mRNA is widely expressed in tissues and in various cell types including platelets, macrophages and endothelial cells.
• Application: Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit has been used in immunoblotting.
• Biochem/physiol Actions: Cytosolic phospholipase A2 (cPLA2) catalyse the synthesis of precursors of proinflammatory mediators, such as prostaglandins and leukotrienes, through the release of arachidonic acid (AA) from membrane phospholipids. PLA2s play crucial roles in several cellular processes, including intracellular membrane trafficking, differentiation, proliferation and apoptosis. They are thought to play a role in oxidative and inflammatory responses in cerebral ischemia, Alzheimer′s disease (AD) and neuronal injury. Upon cell stimulation, cPLA2 is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol to the endoplasmic reticulum and the nuclear membrane. cPLA2 is phosphorylated and activated by either ERK1/2, p38 MAPK or JNK at three sites, Ser505, Ser515 and Ser727, depending on the cell type and agonist.
• Linkage: synthetic peptide corresponding to amino acids 242-258 of human cPLA2 conjugated to KLH. The corresponding sequence is identical in mouse and rat cPLA2
• Physical form: solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.