Anti-MAG Antibody, clone 1I6 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1I6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Myelin-associated glycoprotein (MAG). It targets an epitope within 17 amino acids from the C-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Myelin-associated glycoprotein (MAG).
• Application: Quality Control TestingEvaluated by Western Blotting in Rat brain membrane preparations.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myelin-associated glycoprotein (MAG) in Rat brain membrane preparations.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myelin-associated glycoprotein (MAG) in Human brain and Mouse brain microsomes.Affinity Binding Assay: A representative lot of this antibody bound Myelin-associated glycoprotein (MAG) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Myelin-associated glycoprotein (MAG) in human cerebellum tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Myelin-associated glycoprotein (UniProt: P20916; also known as Siglec-4a, MAG) is encoded by the MAG (also known as GMA) gene (Gene ID: 4099) in human. MAG is a single-pass type I membrane protein that is expressed in myelin-forming cells when oligodendrites initiate contact with axons. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-516), a transmembrane domain (aa 517-536), and a cytoplasmic domain (aa 518-626). MAG is a member of the immunoglobulin superfamily that is found in both peripheral and CNS myelin, where it plays a role in the formation and maintenance of myelin sheath. It is not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. MAG is reported to protect motoneurons against apoptosis and after injury. Protection against apoptosis is probably mediated via interaction with neuronal reticulon receptor 4R (RTN4R) and Reticulon-4 receptor-like 2 protein (RTN4RL2). It is also considered to be important in maintenance of myelin with aging. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Poltorak, M., et al. (1987). J Cell Biol. 105(4); 1893-1899; Quarles, RH. (2007). J. Neurochem. 100(6); 1431-1448).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.