Anti-RIPx/RUFY3 Antibody, clone 1L14 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1L14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects RIPx/RUFY3. It targets an epitope within 20 amino acids from the N-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Protein RIPx/RUFY3.
• Application: Quality Control TestingEvaluated by Western Blotting in Rat brain tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RIPx/RUFY3 in Rat brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected RIPx/RUFY3 in Human brain and Mouse brain tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RIPx/RUFY3 in SH-SY5Y cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RIPx/RUFY3 in human cerebral cortex tissue sections.Affinity Binding Assay: A representative lot of this antibody bound RIPx/RUFY3 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end userEvaluated by Western Blotting in Rat brain tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RIPx/RUFY3 in Rat brain tissue lysate.
• Target description: Protein RUFY3 (UniProt: Q7L099; also known as RUN and FYVE domain-containing protein 3, Rap2-interacting protein x, RIPx, Single axon-regulated protein, Singar) is encoded by the RUFY3 (also known as KIAA0871) gene (Gene ID: 22902) in human. RUFY3 is a RUN domain-containing protein that plays a role in the generation of neuronal polarity formation and axon growth. It is implicated in the formation of a single axon by developing neurons. It may inhibit the formation of additional axons by inhibiting PI-3 kinase in minor neuronal processes. It is reported to specifically interact with actin filament-binding proteins, such as Fascin, and colocalizes with Fascin in growth cones. Knockdown of RUFY3 gene impairs the distribution of Fascin and actin filaments, accompanied by an increased proportion of neurons with multiple axons and a decrease in the axon length. RUFY3 also serves as an adapter protein for small GTPase proteins and is bound to activated Rap2, a Ras family protein in the developing neurons. Overexpression of RUFY3 leads to the formation of F-actin-enriched protrusive structures at the cell periphery and induces gastric cancer cell migration. It is reported that p21-activated kinase-1 (PAK1) interacts with RUFY3 and promotes its expression. Inhibition of PAK1 attenuates RUFY3-incuded cell migration and invasion. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Honda, A., et al. (2017). J. Biol. Chem. 292(51); 20936-20946; Wang, G., et al. (2015). Cell Death Dis. 6(3); e1682; Wei, Z., et al. (2014). J. Neurochem. 130(5); 678-692).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.