Anti-SNRNP40 Antibody, clone 1E15 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1E15 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects U5 small nuclear ribonucleoprotein 40 kDa protein (SNRNP40). It targets an epitope within 16 amino acids from the N-terminal region.
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human U5 small nuclear ribonucleoprotein 40 kDa protein (SNRNP40).
• Application: Quality Control TestingEvaluated by Western Blotting in C6 cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SNRNP40 in C6 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SNRNP40 in lysates from Jurkat cells, Human stomach tissue, and Neuro2A cells.Affinity Binding Assay: A representative lot of this antibody bound SNRNP40 peptide with a KD of 7.8 x 10-7 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SNRNP40 in C6 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SNRNP40 in human small intestine tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: U5 small nuclear ribonucleoprotein 40 kDa protein (UniProt: Q96DI7; also known as U5 snRNP 40 kDa protein; U5-40K, 38 kDa-splicing factor, Prp8-binding protein, hPRP8BP, WD repeat-containing protein 57) is encoded by the SNRNP40 (also known as PRP8BP, SFP38, WDR57) gene (Gene ID: 9410) in human. SNRNP40 is a nuclear protein that is ubiquitously distributed but is strongly expressed in lymphoid tissue. It is a component of the U5 snRNP of the major (U2-dependent) and minor (U-12 dependent) spliceosomes. It is required for pre-mRNA splicing as component of the activated spliceosome, a multiprotein complex that catalyzes the removal of introns from pre-mRNA. It contains seven WD repeats and multiple β strands. SNRNP40 deficiency in primary hematopoietic stem cells, T cells, or the EL4 cell line is reported to increase the frequency of splicing errors, mostly intron retention, in several hundred mRNAs. Two isoforms of SNRNP40 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, D., et al. (2019). Nat. Immunol. 20(10); 1322-1334; Zhan, X., et al. (2018).Cell Res. 28(12); 1129-1140).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
• Reconstitution: 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.