ExtrAvidin®-Agarose, high binding

Description

• General description: ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads.ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
• Application: ExtrAvidin®-Agarose provides high affinity with high specificity binding to Biotin (also known as vitamin B7) including biotinylated proteins and biotin-tagged fusion proteins. The product may be used in various immunological techniques, including Immunoprecipitation and Immunoaffinity purification.
• Caution: DO NOT FREEZE
• Physical form: ExtrAvidin®-Agarose is provided as suspension at a 1:1 ratio, in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
• Legal Information: ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: This product is for research use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.