Endoproteinase Glu-C (V8 Protease)

Description

• General description: Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).
• Specificity: Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.
• Application: Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.[1][2]
• Biochem/physiol Actions: Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.
• Preparation Note: Activator: The enzyme has its maximal activity in presence of SH-reagentsWorking concentration: 1 to 5 mMWorking solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).Storage conditions (working solution): -15 to -25 °CThe enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
• Storage and Stability: Store at 2 to 8 °C. (Store dry!)
• Other Notes: For life science research only. Not for use in diagnostic procedures.