Calpain-1, Porcine Erythrocytes

Description

• General description: Native calpain-1 from porcine erythrocytes. Ca2+-dependent heterodimeric cysteine proteinase with low Ca2+ requirement (EC50= 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin.
• Packaging: Please refer to vial label for lot-specific concentration.
• Warning: Toxicity: Harmful (C)
• Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 9 fluorogenic units
• Physical form: In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
• Reconstitution: Following initial thaw, aliquot and freeze (-70°C). Short-term storage of aliquots at 4°C or on ice is not recommended.
• Other Notes: Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol.81, 323.Sorimachi, H., et al. 1997. Biochem. J.328, 721.Kampfl, A., et al. 1997. J. Neurotrauma14, 121.Johnson, G.V.W., and Gutmann, R.P. 1997. BioEssays19, 1011.Bartus, R.T., et al. 1995. Neurol. Res.17, 249.Wang, K.K.W., and Yuen, P.-W. 1994. Trends Pharmacol. Sci. 15, 412.Saito, K., et al. 1993. Proc. Natl. Acad. Sci. USA90, 2628.Goll, D.E., et al. 1992. BioEssays14, 549.Ishii, H., et al. 1992. Biochim. Biophys. Acta1175, 37.Melloni, E., and Pontremoli, S. 1989. Trends Neurosci.12, 438.Ross, E., and Schatz, G. 1973. Anal. Biochem.54, 304.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany