Calpain-1, Human Erythrocytes

Description

• General description: Native calpain-1 from human erythrocytes. Ca2+-dependent cysteine proteinase with low Ca2+ requirement (half-maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin.
• Packaging: Please refer to vial label for lot-specific concentration.
• Warning: Toxicity: Harmful (C)
• Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit.
• Physical form: In 20 mM imidazole, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
• Preparation Note: Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
• Reconstitution: Following initial thaw, aliquot and freeze (-70°C).
• Analysis Note: Comparable to reference lot by SDS-PAGE
• Other Notes: Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol.81, 323.Sorimachi, H., et al. 1997. Biochem. J. 328, 721.Croall, D.E., and McGrody, K.S. 1994. Biochemistry33, 13223.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany