Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Description

• Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods
• This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution
• This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples
• The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays
• The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer
• Highlights: HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride) Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve Stable – store refrigerated at 4°C for at least one year Includes: 10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer