Thermo Scientific™ Pierce™ DAB Substrate Kit

Description

• Thermo Scientific™ Pierce DAB and the DAB Substrate Kit enable chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in blotting and tissue staining methods
• DAB (3,3'-diaminobenzidine tetrahydrochloride; MW = 214.1) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples
• The brown precipitate is insoluble in alcohol and other organic solvents, making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media
• The Pierce DAB Substrate Kit includes a 10X solution of DAB and Stable Peroxide Substrate Buffer
• Crystalline DAB (powder) is also available
• Highlights: HRP substrate – for detection of horseradish peroxidase activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples Chromogenic – no special equipment needed for visualization; the DAB reaction produces brown bands or spots at sites of reaction with HRP-conjugated antibodies or probes Package options – choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use