Thermo Scientific™ Chloronaphthol Substrate Powder (4-chloro-1-naphthol)

Description

• Thermo Scientific™ Pierce Chloronaphthol (4-CN) Substrates are powders, tablets and ready-made solutions of 4-chloro-1-naphthol for chromogenic detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods
• Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples
• The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB
• However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications
• Highlights: HRP substrate – for detection of horseradish peroxidase activity on solid media Chromogenic – no special equipment needed for visualization Blue-purple – easy to photograph in color or black-and-white Package options – choose powder, tablets, or ready-to-use solution