17-100-57MI

MilliporeSigma™ Upstate™ SMRTα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Manufacturer: MilliporeSigma™

Select a Size

Pack Size SKU Availability Price
Each of 1 17-100-57MI-Each-of-1 In Stock ₹ 53,561.42

17-100-57MI - Each of 1

₹ 53,561.42

In Stock

Quantity

1

Base Price: ₹ 53,561.42

GST (18%): ₹ 9,641.056

Total Price: ₹ 63,202.476

Antigen

SMRTα

Classification

Monoclonal

Formulation

Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C

Gene Symbols

NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1

Immunogen

Recombinant protein corresponding to human SMRT.

Quantity

25 Assays

Research Discipline

Epigenetics and Nuclear Function

Gene ID (Entrez)

NP_001070729.1

Includes

The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.

Content And Storage

-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype

IgG2a κ

Applications

ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Gene Accession No.

Q9Y618

Host Species

Mouse

Purification Method

Unpurified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes SMRT.

Target Species

Human, Mouse, Rat

Form

Ascites

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Description

  • Specifically detects SMRTα Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting The Silencing mediator of retinoic acid & thyroid hormone receptor protein, commonly called the SMRT protein mediates the transcriptional repression of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription machinery
  • Consistent with this activity, this protein is known to form a large corepressor complex containing SIN3A/B and histone deacetylases HDAC1 and HDAC2
  • SMRT is also a component of the N-Cor repressor (nuclear receptor corepressor), a multi-subunit complex minimally composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2
  • SMRT and nuclear receptor corepressor N-CoR are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

Compare Similar Items

Show Difference

Img

MilliporeSigma™

17-100-57MI

--


Antigen:
SMRTα

Classification:
Monoclonal

Formulation:
Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C

Gene Symbols:
NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1

Immunogen:
Recombinant protein corresponding to human SMRT.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_001070729.1

Includes:
The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
IgG2a κ

Applications:
ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q9Y618

Host Species:
Mouse

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes SMRT.

Target Species:
Human, Mouse, Rat

Form:
Ascites

Img

MilliporeSigma™

17-100-61MI

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Antigen:
E2F-1α

Classification:
Monoclonal

Formulation:
Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT

Gene Symbols:
E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3

Immunogen:
GST fusion protein corresponding to full-length human E2F-1.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_005216

Includes:
This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
IgG

Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation

Conjugate:
Unconjugated

Gene Accession No.:
Q01094

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.

Target Species:
Human, Xenopus species

Form:
Purified

Img

MilliporeSigma™

17-100-71

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Classification:
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Formulation:
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Immunogen:
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MilliporeSigma™

17-100-77

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Antigen:
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Classification:
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Formulation:
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Immunogen:
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Quantity:
96 Assays

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Includes:
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Content And Storage:
Store components at the temperatures indicated on the labels

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Applications:
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Conjugate:
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Gene Accession No.:
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Host Species:
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Purification Method:
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Regulatory Status:
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Primary or Secondary:
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Form:
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