IRF7 Monoclonal Antibody (RDP4ND4), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody RDP4ND4 recognizes human interferon regulatory factor 7 (IRF7), a 54 kDa member of the interferon regulatory transcription factor family
• It is essential for the antiviral innate immune response and the activation of type I IFN genes
• IRF7 is constitutively expressed in the cytoplasm of plasmacytoid dendritic cells, B cells and monocytes, but it is strongly induced in response to virus infection and interferon stimulation
• IRF7 can activate IFN-beta and IFN-alpha genes via the virus-activated, MyD88-independent pathway, or the TLR-activated, MyD88-dependent pathway
• Virus exposure induces IRF7, through toll-like receptors TLR7, TLR8, and TLR9, to promote IFN gene expression and disrupt infection
• IRF7 exists in an inactive form until phosphorylated and its phosphorylation leads to dimerization and nuclear translocation of IRF7 where it aids in activating target genes
• The IRF7 gene is upregulated by interferon stimulation through the Jak-STAT pathway in a positive feedback loop
• Applications Reported: This RDP4ND4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RDP4ND4 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No
• 00-5523) and protocol
• Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms
• During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes
• IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes
• IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling
• There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.