IRF3 Monoclonal Antibody (SL-12 (SL12)), eBioscience™, Invitrogen™

Description

• Description: This SL-12 monoclonal antibody reacts with human Interferon Regulatory Factor (IRF)-3
• IRF-3 is a 427 amino acid constitutively expressed member of the IRF transcription factor family that plays a critical role in the induction of type I interferons
• Inactive IRF-3 continuously transits between the cytoplasm and nucleus
• Virus or dsRNA induces the C-terminal phosphorylation of IRF-3 by IKKε and TBK-1
• This phosphorylation results in retention of IRF-3 in the nucleus and subsequent transcription of IFN-β, several interferon stimulated genes (ISGs), and other antiviral response genes
• Applications Reported: This SL-12 (SL12) antibody has been reported for use in immunoblotting (WB)
• Applications Tested: This SL-12 (SL12) antibody has been tested by immunoblot of normal human peripheral blood cell lysates
• This antibody can be used at less than or equal to 2 μg/mL in blocking buffer
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses
• It plays a critical role in the innate immune response against DNA and RNA viruses
• IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters
• It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction
• IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling
• It is phosphorylated by IKBKE and TBK1 kinases
• This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes
• Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.