14-999-482
Perforin Monoclonal Antibody (dG9 (delta G9)), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 14-999-482-Each-of-1 | In Stock | ₹ 28,234.80 |
14-999-482 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 28,234.80
GST (18%): ₹ 5,082.264
Total Price: ₹ 33,317.064
Antigen
Perforin
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P14222
Gene Symbols
PRF1
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
396595, 5551
Content And Storage
4° C
Form
Liquid
Applications
Immunohistochemistry, Immunoprecipitation
Clone
dG9 (delta G9)
Conjugate
Unconjugated
Gene
PRF1
Gene Alias
Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Porcine
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp)
- Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK)
- Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections
- Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
- (Fluorochrome conjugated dG9 (delta G9) is recommended for use in intracellular flow cytometry.)
- Applications Tested: This dG9 (delta G9) antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- Perforin is one of the major cytolytic proteins of cytolytic granules
- Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules
- Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes
- Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD
- Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated
- Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested
- Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis
- Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood
- Alternative splicing results in multiple transcript variants of perforin.
Compare Similar Items
Show Difference
Antigen: Perforin
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P14222
Gene Symbols: PRF1
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 396595, 5551
Content And Storage: 4° C
Form: Liquid
Applications: Immunohistochemistry, Immunoprecipitation
Clone: dG9 (delta G9)
Conjugate: Unconjugated
Gene: PRF1
Gene Alias: Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human, Porcine
Product Type: Antibody
Isotype: IgG2b κ
Antigen:
Perforin
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P14222
Gene Symbols:
PRF1
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
396595, 5551
Content And Storage:
4° C
Form:
Liquid
Applications:
Immunohistochemistry, Immunoprecipitation
Clone:
dG9 (delta G9)
Conjugate:
Unconjugated
Gene:
PRF1
Gene Alias:
Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human, Porcine
Product Type:
Antibody
Isotype:
IgG2b κ
Antigen: IgM
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P01871
Gene Symbols: IGHM
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 3507
Content And Storage: 4° C
Form: Liquid
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen)
Clone: SA-DA4
Conjugate: Unconjugated
Gene: IGHM
Gene Alias: AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH
Host Species: Mouse
Quantity: 2 mg
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
IgM
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P01871
Gene Symbols:
IGHM
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
3507
Content And Storage:
4° C
Form:
Liquid
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Frozen)
Clone:
SA-DA4
Conjugate:
Unconjugated
Gene:
IGHM
Gene Alias:
AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH
Host Species:
Mouse
Quantity:
2 mg
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: __
Classification: __
Concentration: 2X
Formulation: __
Gene Accession No.: __
Gene Symbols: __
Purification Method: __
Regulatory Status: __
Gene ID (Entrez): __
Content And Storage: • Platinum II PCR Master Mix (2X), 1.25 mL• Platinum GC Enhancer, 1.25 mL• Nuclease-free water, 1.25 mLStore at -20°C in a non-frost-free freezer.
Form: __
Applications: __
Clone: __
Conjugate: __
Gene: __
Gene Alias: __
Host Species: __
Quantity: __
Primary or Secondary: __
Target Species: __
Product Type: Hot Start PCR Master Mix
Isotype: __
Antigen:
__
Classification:
__
Concentration:
2X
Formulation:
__
Gene Accession No.:
__
Gene Symbols:
__
Purification Method:
__
Regulatory Status:
__
Gene ID (Entrez):
__
Content And Storage:
• Platinum II PCR Master Mix (2X), 1.25 mL• Platinum GC Enhancer, 1.25 mL• Nuclease-free water, 1.25 mLStore at -20°C in a non-frost-free freezer.
Form:
__
Applications:
__
Clone:
__
Conjugate:
__
Gene:
__
Gene Alias:
__
Host Species:
__
Quantity:
__
Primary or Secondary:
__
Target Species:
__
Product Type:
Hot Start PCR Master Mix
Isotype:
__
Antigen: __
Classification: __
Concentration: 2X
Formulation: __
Gene Accession No.: __
Gene Symbols: __
Purification Method: __
Regulatory Status: __
Gene ID (Entrez): __
Content And Storage: • Platinum II Green PCR Master Mix (2X), 1.25 mL• Platinum GC Enhancer, 1.25 mL• Nuclease-free water, 1.25 mLStore at -20°C in a non-frost-free freezer.
Form: __
Applications: __
Clone: __
Conjugate: __
Gene: __
Gene Alias: __
Host Species: __
Quantity: __
Primary or Secondary: __
Target Species: __
Product Type: Hot Start PCR Master Mix
Isotype: __
Antigen:
__
Classification:
__
Concentration:
2X
Formulation:
__
Gene Accession No.:
__
Gene Symbols:
__
Purification Method:
__
Regulatory Status:
__
Gene ID (Entrez):
__
Content And Storage:
• Platinum II Green PCR Master Mix (2X), 1.25 mL• Platinum GC Enhancer, 1.25 mL• Nuclease-free water, 1.25 mLStore at -20°C in a non-frost-free freezer.
Form:
__
Applications:
__
Clone:
__
Conjugate:
__
Gene:
__
Gene Alias:
__
Host Species:
__
Quantity:
__
Primary or Secondary:
__
Target Species:
__
Product Type:
Hot Start PCR Master Mix
Isotype:
__