TCR V beta F1 Monoclonal Antibody (8A3), APC, eBioscience™, Invitrogen™

Description

• Description: This 8A3 monoclonal antibody recognizes human TCR beta F1, a common framework determinant on the beta-subunit of the T cell receptor
• Expression of TCR beta F1 has been described as a potential diagnostic marker indicating a favorable outcome for tumor patients with not otherwise specified Nodal Peripheral T-cell Lymphoma (PTCL-NOS)
• Furthermore, a lack of surface and cytoplasmic TCR beta F1 was also found in small cell variant of T-cell prolymphocytic Leukemia
• This antibody will not stain the native form of the TCR and will require fixing and permeabilizing cells
• Applications Reported: This 8A3 antibody has been reported for use in flow cytometric analysis, immunofluorescent staining of frozen tissue sections, and immunocytochemistry
• Applications Tested: This 8A3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser The T Cell Receptor alpha beta (TCRab) is a heterodimeric membrane receptor that consists of the highly variable alpha and beta chains expressed as part of a complex with the invariant CD3 proteins
• TCRab participates in the activation of T-cells in response to an antigen presented in the context of MHC
• Engagement of the TCRab initiates signaling cascades that μgtimately result in cellular proliferation, differentiation, cytokine production, and/or activation-induced cell death
• These signaling cascades regulate T-cell development, homeostasis, activation, acquisition of effector functions and apoptosis
• Cells expressing TCRab constitute the vast majority of T cells in both human and mouse.