200064693
CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Brilliant Ultra Violet™ 661, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD279 (PD-1)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
eBioJ105 (J105)
Applications
Flow Cytometry
Conjugate
Brilliant Ultraviolet 661
Host Species
Mouse
Target Species
Human, Rhesus Monkey
Gene Accession No.
Q15116
Gene ID (Entrez)
100135775, 5133
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Pdcd1
Related Products
Description
- CD279 (PD-1) Monoclonal antibody specifically detects CD279 (PD-1) in Human, Rhesus Monkey samples
- It is validated for Flow Cytometry Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily
- PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease
- PD-1 is expressed predominantly on activated T and B lymphocytes
- Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC)
- Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells
- Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat
- 11-9969)
- Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBioJ105 (J105) antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye that emits at 660 nm and is intended for use on cytometers equipped with an μgtraviolet (355 nm) laser
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
- Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
- Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet Laser
- BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
- Powered by Sirigen.™
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Antigen: CD279 (PD-1)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: eBioJ105 (J105)
Applications: Flow Cytometry
Conjugate: Brilliant Ultraviolet 661
Host Species: Mouse
Target Species: Human, Rhesus Monkey
Gene Accession No.: Q15116
Gene ID (Entrez): 100135775, 5133
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Pdcd1
Antigen:
CD279 (PD-1)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
eBioJ105 (J105)
Applications:
Flow Cytometry
Conjugate:
Brilliant Ultraviolet 661
Host Species:
Mouse
Target Species:
Human, Rhesus Monkey
Gene Accession No.:
Q15116
Gene ID (Entrez):
100135775, 5133
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Pdcd1
Antigen: Collagen IV
Concentration: 1 mg/mL
Classification: Recombinant Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: [a]3(IV); [a]4(IV); alpha 1 type IV collagen; alpha type IV collagen basement membrane; alpha1(IV) collagen; alpha-2 collagen type IV; alpha-3 type IV collagen; alpha3(IV); Arresten; Bru; BSVD; CA44; Canstatin; Col4a1; Col4a-1; COL4A1 NC1 domain; Col4a2; Col4a-2; COL4A3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha 1 chain; collagen type IV alpha 2; collagen type IV alpha 2 chain; collagen type IV alpha 3 chain; collagen type IV alpha 4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)44H; Del(8)Bru44H; E130010M05Rik; Goodpasture antigen; ICH; LOW QUALITY PROTEIN: collagen alpha-1(IV) chain; LOW QUALITY PROTEIN: collagen alpha-3(IV) chain; LOW QUALITY PROTEIN: collagen alpha-4(IV) chain; POREN2; procollagen alpha1(IV); procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; RATOR; Raw; retinal anterior wiring; Svc; tumstatin; Type IV Collagen
Isotype: IgG κ
Purification Method: Protein A
Product Type: Antibody
Clone: COL4/9395R
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: P02462, P08572, P29400, P53420, Q01955
Gene ID (Entrez): 1282, 1284, 1285, 1286, 1287
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: __
Antigen:
Collagen IV
Concentration:
1 mg/mL
Classification:
Recombinant Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
[a]3(IV); [a]4(IV); alpha 1 type IV collagen; alpha type IV collagen basement membrane; alpha1(IV) collagen; alpha-2 collagen type IV; alpha-3 type IV collagen; alpha3(IV); Arresten; Bru; BSVD; CA44; Canstatin; Col4a1; Col4a-1; COL4A1 NC1 domain; Col4a2; Col4a-2; COL4A3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha 1 chain; collagen type IV alpha 2; collagen type IV alpha 2 chain; collagen type IV alpha 3 chain; collagen type IV alpha 4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)44H; Del(8)Bru44H; E130010M05Rik; Goodpasture antigen; ICH; LOW QUALITY PROTEIN: collagen alpha-1(IV) chain; LOW QUALITY PROTEIN: collagen alpha-3(IV) chain; LOW QUALITY PROTEIN: collagen alpha-4(IV) chain; POREN2; procollagen alpha1(IV); procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; RATOR; Raw; retinal anterior wiring; Svc; tumstatin; Type IV Collagen
Isotype:
IgG κ
Purification Method:
Protein A
Product Type:
Antibody
Clone:
COL4/9395R
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
P02462, P08572, P29400, P53420, Q01955
Gene ID (Entrez):
1282, 1284, 1285, 1286, 1287
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
__
Antigen: Collagen II (COL2A1)
Concentration: 200 μg/mL
Classification: Recombinant Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha 1 type IIA collagen precursor; alpha collagen type II; alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CB11; CG2A1A; CGA; Chondrocalcin; COL11A3; Col2; Col2a; col2a1; Col2a-1; col2a1.L; col2a1a; col2a1-a; col2a1-b; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen type II alpha 1 chain; collagen type IIA; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); collagen, type II, alpha 1 L homeolog; COLLII; cyanogen bromide; Del1; disproportionate micromelia; Dmm; Lpk; M100856; MGC131516; pro alpha1 type II collagen; pro-alpha 1(II); pro-alpha-1 type II collagen; procollagen alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; Rgsc856; SEDC; STL1; type II collagen; type II collagen cyanogen bromide fragment CB10; type II collogen cyanogen bromide fragment CB8; unnamed protein product; X-col II; xcol2a1; XELAEV_18013523mg
Isotype: IgG κ
Purification Method: Protein A/G
Product Type: Antibody
Clone: COL2A1/8882R
Applications: ELISA, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: P02458
Gene ID (Entrez): 1280
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Col2a1
Antigen:
Collagen II (COL2A1)
Concentration:
200 μg/mL
Classification:
Recombinant Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha 1 type IIA collagen precursor; alpha collagen type II; alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CB11; CG2A1A; CGA; Chondrocalcin; COL11A3; Col2; Col2a; col2a1; Col2a-1; col2a1.L; col2a1a; col2a1-a; col2a1-b; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen type II alpha 1 chain; collagen type IIA; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); collagen, type II, alpha 1 L homeolog; COLLII; cyanogen bromide; Del1; disproportionate micromelia; Dmm; Lpk; M100856; MGC131516; pro alpha1 type II collagen; pro-alpha 1(II); pro-alpha-1 type II collagen; procollagen alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; Rgsc856; SEDC; STL1; type II collagen; type II collagen cyanogen bromide fragment CB10; type II collogen cyanogen bromide fragment CB8; unnamed protein product; X-col II; xcol2a1; XELAEV_18013523mg
Isotype:
IgG κ
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
COL2A1/8882R
Applications:
ELISA, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
P02458
Gene ID (Entrez):
1280
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Col2a1
Antigen: Collagen II (COL2A1)
Concentration: 200 μg/mL
Classification: Recombinant Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha 1 type IIA collagen precursor; alpha collagen type II; alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CB11; CG2A1A; CGA; Chondrocalcin; COL11A3; Col2; Col2a; col2a1; Col2a-1; col2a1.L; col2a1a; col2a1-a; col2a1-b; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen type II alpha 1 chain; collagen type IIA; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); collagen, type II, alpha 1 L homeolog; COLLII; cyanogen bromide; Del1; disproportionate micromelia; Dmm; Lpk; M100856; MGC131516; pro alpha1 type II collagen; pro-alpha 1(II); pro-alpha-1 type II collagen; procollagen alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; Rgsc856; SEDC; STL1; type II collagen; type II collagen cyanogen bromide fragment CB10; type II collogen cyanogen bromide fragment CB8; unnamed protein product; X-col II; xcol2a1; XELAEV_18013523mg
Isotype: IgG κ
Purification Method: Protein A/G
Product Type: Antibody
Clone: COL2A1/8810R
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: P02458
Gene ID (Entrez): 1280
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Col2a1
Antigen:
Collagen II (COL2A1)
Concentration:
200 μg/mL
Classification:
Recombinant Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha 1 type IIA collagen precursor; alpha collagen type II; alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CB11; CG2A1A; CGA; Chondrocalcin; COL11A3; Col2; Col2a; col2a1; Col2a-1; col2a1.L; col2a1a; col2a1-a; col2a1-b; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen type II alpha 1 chain; collagen type IIA; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); collagen, type II, alpha 1 L homeolog; COLLII; cyanogen bromide; Del1; disproportionate micromelia; Dmm; Lpk; M100856; MGC131516; pro alpha1 type II collagen; pro-alpha 1(II); pro-alpha-1 type II collagen; procollagen alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; Rgsc856; SEDC; STL1; type II collagen; type II collagen cyanogen bromide fragment CB10; type II collogen cyanogen bromide fragment CB8; unnamed protein product; X-col II; xcol2a1; XELAEV_18013523mg
Isotype:
IgG κ
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
COL2A1/8810R
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
P02458
Gene ID (Entrez):
1280
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Col2a1