Axl Monoclonal Antibody (MAXL8DS), Brilliant Violet™ 711, eBioscience™, Invitrogen™

Description

• Axl Monoclonal antibody specifically detects Axl in Mouse samples
• It is validated for Flow Cytometry Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3
• Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages
• Stimulation with TLR ligands has been shown to additionally upregulate this expression
• This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells
• Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Brilliant Violet™ 711 (BV711) is a tandem dye that emits at 713 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href='https://www.thermofisher.com/order/catalog/product/SB-4401-42'>Product # SB-4401-42</a>) or Brilliant Stain Buffer™ (<a href='https://www.thermofisher.com/order/catalog/product/00-4409-75'>Product # 00-4409-75</a>) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (<a href='https://www.thermofisher.com/order/catalog/product/00-8222-49'>Product # 00-8222-49</a>) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href='https://www.thermofisher.com/order/catalog/product/00-5333-54'>Product # 00-5333-54</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
• Our internal testing suggests that Brilliant Violet™ 711 (BV711) is not compatible with methanol-based fixation
• Excitation: 407 nm; Emission: 713 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
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