CD366 (TIM3) Monoclonal Antibody (RMT3-23), Brilliant Violet™ 421, eBioscience™, Invitrogen™

Description

• CD366 (TIM3) Monoclonal antibody specifically detects CD366 (TIM3) in Mouse samples
• It is validated for Flow Cytometry Description: The RMT3-23 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein
• The RMT3-23 antibody reacts with CD366 protein expressed by both BALB/c and C57BL/6 strains of mice
• CD366, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion
• CD366 is expressed selectively by differentiated CD4+ Th1 and CD8+ Tc1 cells, but is absent on CD4+Th2 and CD8+ Tc2 cells
• Other hematopoietic cell types, including naive T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level
• CD366 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation
• In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed CD366 after immunization
• RMT3-23 has been shown to have functional activity; blocks DC recognition of apoptotic cells and also induces autoantibody production
• Applications Reported: This RMT3-23 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RMT3-23 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href='https://www.thermofisher.com/order/catalog/product/SB-4401-42'>Product # SB-4401-42</a>) or Brilliant Stain Buffer™ (<a href='https://www.thermofisher.com/order/catalog/product/00-4409-75'>Product # 00-4409-75</a>) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
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