Axl Monoclonal Antibody (DS7HAXL), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3
• This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells
• It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis
• Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis
• Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma
• The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation
• TAM receptors are also involved in phagocytosis of apoptotic cells
• Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies
• In addition, Axl may function as a putative entry receptor for filoviruses
• Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells
• Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C
• Soluble Axl is generated by proteolytic cleavage of the membrane form
• Increased plasma levels may be indicative of inflammation and cancer.