Adaptin α Mouse, Unlabeled, Clone: 8, BD

Description

• Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles
• Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats
• These coatomers contain two large Adaptin proteins (α, γ, δ, or ε and β1, β2, β3 or β4, respectively) that are noncovalently linked to one medium chain (μ1, μ2, μ3 or μ4) and one small chain (σ1, σ2, σ3 or σ4)
• The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis
• In the AP-2 complex, Adaptin α is expressed in two very similar isoforms from two different genes
• Adaptin α [A] (112kDa) is expressed primarily in brain, while Adaptin α [C] (105kDa) is expressed in brain, liver and other tissues
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting