IL-23 p19 Monoclonal Antibody (fc23cpg), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• This fc23cpg MAb reacts with the p19 subunit of mouse IL-23, a heterodimeric cytokine made up of two disulfide-linked subunits, p40 and p19
• It is closely related to IL-12, with which it shares the p40 subunit
• IL-23 signals through a heterodimeric receptor expressed on activated T cells, macrophages, and dendritic cells
• The receptor shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex
• Binding of IL-23 to its receptor activates Jak/STAT pathways similarly to IL-12, but the two cytokines exhibit distinct functions
• Unlike IL-12, which induces T-bet and Th1 differentiation, IL-23 signaling results in RORgammat expression and supports CD4^+ T cells with the Th17 phenotype
• Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the beta-glucan receptor, Dectin-1
• Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels
• Recent publications suggest the p19 subunit may also exist in the absence of association with p40
• IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19
• p19, like p35 of IL-12, is biologically inactive by itself
• IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R
• IL-23 is secreted by activated mouse and human dendritic cells
• Biological activities of mouse IL-23 are distinct from those of mouse IL-12
• Mouse IL-23 was found not to induce significant amounts of IFN-γ
• Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells
• Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17
• Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.