MICA/B Monoclonal Antibody (6D4), PE, eBioscience™, Invitrogen™

Description

• Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins
• MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules
• The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I
• MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells
• There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB
• Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney
• Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A
• The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin
• It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.