Galectin 9 Monoclonal Antibody (9M1-3), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This 9M1-3 monoclonal antibody reacts with human Galectin-9
• Galectin-9 is one of fifteen members of the galectin family of glycan-binding proteins
• It is expressed on activated T helper cells and Foxp3+ T regulatory cells and plays a critical role in the regulation of immune function
• Interaction of Galectin-9 with its ligand, TIM3, induces apoptosis in Th1 and Th17, inhibits Th17 polarization and IFN gamma expression, and drives Treg expansion
• Galectin-9 is also thought to contribute to the survival and expansion of some tumors by inhibiting the anti-tumor response and promoting immune evasion
• Along with Galectin-1 and Galectin-3, high levels of Galectin-9 expression are associated with poor cancer prognosis, making these potential therapeutic targets
• Applications Reported: This 9M1-3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 9M1-3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions
• The protein encoded by this gene is an S-type lectin
• It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency
• Multiple alternatively spliced transcript variants have been found for this gene.