OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), eBioscience™, Invitrogen™

Description

• Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide
• This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo
• Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
• Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1
• With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM
• 2
• Incubate cells at 37°C for 2 hours
• 3
• Wash cells with flow staining buffer
• 4
• Proceed with cell surface staining as normal
• For additional information see the references listed below
• Purity: Greater than 90%, as determined by SDS-PAGE
• OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples
• It is validated for Flow Cytometry, Immunohistochemistry.