CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells
• CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4
• However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain
• This results in a 54 kDa fragment containing all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains
• The 54 kDa can remain membrane-associated or be released as soluble CD223
• CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation
• Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function
• Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA
• The functional grade eBioC9B7W has been reported for the in vitro and in vivo blocking of CD223 function
• Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of anti-CD3 and anti-CD28-activated mouse splenocytes
• This can be used at less than or equal to 0
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).