B7-H4 Monoclonal Antibody (H74), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x
• Cross reactivity of this antibody to other proteins has not been determined
• B7S1 is a newly discovered member of the B7 family
• It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells
• While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand
• H74 stains human B7-H4 transfected cells and not peripheral blood cells
• Exact expression pattern of B7-H4 has not been fully characterized
• Applications Reported: This H74 antibody has been reported for use in flow cytometric analysis
• Applicability of this antibody in in vitro assays for blocking or activation has not been determined
• Applications Tested: This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• Storage and handling: Use in a sterile environment
• B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity
• Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production
• B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response
• B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells
• Aberrant expression has been associated with cancers of the lung, breast and ovary in humans
• B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses
• Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression
• Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).