B7-H4 Monoclonal Antibody (188), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X
• Cross reactivity of this antibody to other proteins has not been determined
• B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production
• The ligand for B7-H4 has not been identified yet
• Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, Clone 9 and 188, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together
• 188 stains mouse B7-H4 transfected cells and not spleen cells
• Exact expression pattern of B7-H4 has not been fully characterized
• Applications Reported: This 188 antibody has been reported for use in flow cytometric analysis and has also been reported to block binding of B7-H4 ligand in vitro
• Applications Tested: The 188 antibody has been tested by flow cytometric analysis of transfected cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity
• Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production
• B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response
• B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells
• Aberrant expression has been associated with cancers of the lung, breast and ovary in humans
• B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses
• Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression
• Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).