CD244 Monoclonal Antibody (eBioDM244), FITC, eBioscience™, Invitrogen™

Description

• Description: The eBioDM244 monoclonal antibody reacts with human CD244 (2B4, p38)
• In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gamma delta T cells, monocytes, basophils and eosinophils
• Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor
• Applications Reported: This eBioDM244 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioDM244 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing
• The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity
• CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells
• Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease
• Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome
• Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.