CD66a (CEACAM1) Monoclonal Antibody (CC1), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody CC1 recognizes CD66a, also known as carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), biliary glycoprotein, and BPG
• Expression of CD66a is found on brush borders, epithelial, and endothelial cells
• In hematopoietic cells expression is found abundantly on B cells, as well as some NKs, monocytes, DCs, and granulocytes
• Although low levels of mRNA have been identified in T cells in humans, resting mouse T lymphocytes are not reported to express CD66a, as confirmed by lack of staining with CC1 antibody
• In humans, expression levels of CD66a have been used to identify maligcies
• CD66a plays a key role as a regulator of BCR activation of B lymphocytes
• An alternate allele, CEACAM1b, is expressed in SJL mice; therefore, CC1 does not stain SJL tissue
• The monoclonal CC1 has been shown to block viral infection and also enhance B cell proliferation when combined with IgM crosslinking
• Applications Reported: This CC1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• CEACAM1 is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily
• Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19
• Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster
• The encoded protein was originally described in bile ducts of liver as biliary glycoprotein
• Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia
• The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup
• Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses
• Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined.