LAP (Latency Associated peptide) Monoclonal Antibody (TW7-16B4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The TW7-16B4 monoclonal antibody reacts with mouse latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1)
• Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types
• TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus
• Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex
• LAP is secreted and can be found in the extracellular matrix
• In addition, LAP can also be expressed on platelets and activated regulatory T cells
• It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells
• Applications Reported: This TW7-16B4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This TW7-16B4 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues
• Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain
• Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins
• Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.