5539908

VCAM-1/CD106 Antibody (B-K9), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

VCAM-1/CD106

Concentration

0.2 mg/mL

Applications

Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

Gene Symbols

VCAM1

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Clone

B-K9

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P19320

Gene ID (Entrez)

7412

Immunogen

Activated human umbilical vein endothelial cells (HUVEC)

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

110 kDa

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence VCAM-1/CD106 Monoclonal specifically detects VCAM-1/CD106 in Human, Rat samples
  • It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence.

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Antigen:
VCAM-1/CD106

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

Gene Symbols:
VCAM1

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Clone:
B-K9

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P19320

Gene ID (Entrez):
7412

Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
110 kDa

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Antigen:
Vimentin

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
FLJ36605, vimentin

Gene Symbols:
VIM

Isotype:
IgM

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Clone:
LN-6

Dilution:
Flow Cytometry 0.5 - 1.0 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Feline, Primate, Rabbit, Sheep

Gene Accession No.:
P08670

Gene ID (Entrez):
7431

Immunogen:
Human thymic nuclear extract

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__

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Antigen:
Von Willebrand Factor

Concentration:
0.2 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Gene Symbols:
VWF

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Clone:
IIIE2.34

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunoprecipitation 0.5 - 1 ug/500 ug Protein Lysate, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P04275

Gene ID (Entrez):
7450

Immunogen:
Recombinant human vWF fragment spanning aa 845-949

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
250 kDa

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5539931

Mouse Monoclonal Antibody...


Antigen:
Von Willebrand Factor

Concentration:
0.2 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Gene Symbols:
VWF

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Clone:
IIIE2.34

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunoprecipitation 0.5 - 1 ug/500 ug Protein Lysate, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P04275

Gene ID (Entrez):
7450

Immunogen:
Recombinant human vWF fragment spanning aa 845-949

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
250 kDa