5539925

Vimentin Antibody (LN-6), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Vimentin

Concentration

0.2mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

FLJ36605, vimentin

Gene Symbols

VIM

Isotype

IgM

Purification Method

Protein A or G purified

Test Specificity

This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Clone

LN-6

Dilution

Flow Cytometry 0.5 - 1.0 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Feline, Primate, Rabbit, Sheep

Gene Accession No.

P08670

Gene ID (Entrez)

7431

Immunogen

Human thymic nuclear extract

Primary or Secondary

Primary

Content And Storage

Store at 4C.

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Vimentin Monoclonal specifically detects Vimentin in Human, Mouse, Rat, Porcine, Bovine, Feline, Rabbit, Sheep samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

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Fischer Scientific

5539925

Mouse Monoclonal Antibody has been ...


Antigen:
Vimentin

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
FLJ36605, vimentin

Gene Symbols:
VIM

Isotype:
IgM

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Clone:
LN-6

Dilution:
Flow Cytometry 0.5 - 1.0 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Feline, Primate, Rabbit, Sheep

Gene Accession No.:
P08670

Gene ID (Entrez):
7431

Immunogen:
Human thymic nuclear extract

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Img

Fischer Scientific

5539931

Mouse Monoclonal Antibody...


Antigen:
Von Willebrand Factor

Concentration:
0.2 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Gene Symbols:
VWF

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Clone:
IIIE2.34

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunoprecipitation 0.5 - 1 ug/500 ug Protein Lysate, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P04275

Gene ID (Entrez):
7450

Immunogen:
Recombinant human vWF fragment spanning aa 845-949

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Img

Fischer Scientific

5539931

Mouse Monoclonal Antibody...


Antigen:
Von Willebrand Factor

Concentration:
0.2 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Gene Symbols:
VWF

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Clone:
IIIE2.34

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunoprecipitation 0.5 - 1 ug/500 ug Protein Lysate, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P04275

Gene ID (Entrez):
7450

Immunogen:
Recombinant human vWF fragment spanning aa 845-949

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

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Fischer Scientific

5539943

Mouse Monoclonal Antibody...


Antigen:
WT1

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Angiogenesis, Cancer, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Tumor Suppressors

Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
AWT1, NPHS4GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT-2, WT33

Gene Symbols:
WT1

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for

Clone:
WT1/857

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat

Gene Accession No.:
P19544

Gene ID (Entrez):
7490

Immunogen:
Recombinant human WT1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.