5540249

S100A9 Antibody (CPT/1028), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

S100A9

Concentration

0.2 mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases

Formulation

1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez)

6280

Immunogen

Recombinant human Calprotectin protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

14 kDa

Clone

CPT/1028

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)

Gene Symbols

S100A9

Isotype

IgM κ

Purification Method

Protein A or G purified

Test Specificity

Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infe

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunohistochemistry (Frozen) (Negative) S100A9 Monoclonal specifically detects S100A9 in Human samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

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Fischer Scientific

5540249

Mouse Monoclonal Antibody...


Antigen:
S100A9

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases

Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
6280

Immunogen:
Recombinant human Calprotectin protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
14 kDa

Clone:
CPT/1028

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)

Gene Symbols:
S100A9

Isotype:
IgM κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infe

Img

Fischer Scientific

5540250

Mouse Monoclonal Antibody...


Antigen:
S100A9

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases

Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
6280

Immunogen:
Recombinant human MRP14 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
14 kDa

Clone:
MRP14/840

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)

Gene Symbols:
__

Isotype:
IgM κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of14kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This MAb reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue s

Img

Fischer Scientific

5540251

Mouse Monoclonal Antibody...


Antigen:
S100A9

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases

Formulation:
10mM PBS with 0.05% Sodium Azide

Gene ID (Entrez):
6280

Immunogen:
Recombinant human S100A9 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
14 kDa

Clone:
S100A9/1011

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)

Gene Symbols:
S100A9

Isotype:
IgM κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cel

Img

Fischer Scientific

5540251

Mouse Monoclonal Antibody...


Antigen:
S100A9

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases

Formulation:
10mM PBS with 0.05% Sodium Azide

Gene ID (Entrez):
6280

Immunogen:
Recombinant human S100A9 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
14 kDa

Clone:
S100A9/1011

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)

Gene Symbols:
S100A9

Isotype:
IgM κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cel