5545290
PMEL17/SILV Antibody (HMB45), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 3 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
PMEL17/SILV
Concentration
0.2mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human, Canine (Negative), Rat (Negative)
Gene Alias
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols
PMEL
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Clone
HMB45
Dilution
Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:50 - 1:100
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez)
6490
Immunogen
Extract of pigmented melanoma metastases from lymph nodes
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
95 kDa
Related Products
Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence PMEL17/SILV Monoclonal specifically detects PMEL17/SILV in Human, Canine (Negative), Rat (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular), Immunofluorescence, Multiplex Immunoassay.
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Antigen: PMEL17/SILV
Concentration: 0.2mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Canine (Negative), Rat (Negative)
Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols: PMEL
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Clone: HMB45
Dilution: Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:50 - 1:100
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 6490
Immunogen: Extract of pigmented melanoma metastases from lymph nodes
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 95 kDa
Antigen:
PMEL17/SILV
Concentration:
0.2mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Canine (Negative), Rat (Negative)
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols:
PMEL
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Clone:
HMB45
Dilution:
Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:50 - 1:100
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
6490
Immunogen:
Extract of pigmented melanoma metastases from lymph nodes
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
95 kDa
Antigen: HLA DQ
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Porcine
Gene Alias: CD, CELIAC1DQ alpha 1 chain, DC-1 alpha chain, DQ-A1, FLJ27088, FLJ27328, GSE, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, HLA-DQA, leucocyte antigen DQA1, leukocyte antigen alpha chain, major histocompatibility complex, class II, DQ alpha 1, MGC149527, MHC class II antigen, MHC class II DQA1, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II surface glycoprotein, MHC HLA-DQ alpha
Gene Symbols: HLA-DQ
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.
Clone: SPV-L3
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 3117
Immunogen: T4-positive CTL clone HG-38
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 60 kDa
Antigen:
HLA DQ
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Porcine
Gene Alias:
CD, CELIAC1DQ alpha 1 chain, DC-1 alpha chain, DQ-A1, FLJ27088, FLJ27328, GSE, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, HLA-DQA, leucocyte antigen DQA1, leukocyte antigen alpha chain, major histocompatibility complex, class II, DQ alpha 1, MGC149527, MHC class II antigen, MHC class II DQA1, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II surface glycoprotein, MHC HLA-DQ alpha
Gene Symbols:
HLA-DQ
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.
Clone:
SPV-L3
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
3117
Immunogen:
T4-positive CTL clone HG-38
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
60 kDa
Antigen: EpCAM/TROP1
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Rat (Negative)
Gene Alias: 17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
Gene Symbols: EPCAM
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Clone: MOC-31
Dilution: Western Blot 1:100 - 1:200, Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100 - 1:200, SDS-Page, Immunofluorescence 1:50 - 1:100
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 4072
Immunogen: Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 41 kDa
Antigen:
EpCAM/TROP1
Concentration:
0.2mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Rat (Negative)
Gene Alias:
17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
Gene Symbols:
EPCAM
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Clone:
MOC-31
Dilution:
Western Blot 1:100 - 1:200, Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100 - 1:200, SDS-Page, Immunofluorescence 1:50 - 1:100
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
4072
Immunogen:
Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
41 kDa
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