CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells
• Applications Reported: This HIR2 (GA-R2) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HIR2 (GA-R2) antibody has been pre-diluted and tested by flow cytometric analysis of diluted whole blood
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells
• CD235a is the carrier of blood group M and N specificities
• CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation
• CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.