Collagen X Monoclonal Antibody (X53), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody X53 recognizes human, mouse, porcine, guinea pig, and rat Collagen Type X
• X53 does not cross-react with collagen types I, II, III, IV, or VI
• Collagen Type X is a homotrimeric short chain collagen
• Collagen Type X has limited expression in normal adult tissues, primarily being expressed during bone growth and development
• Expression is localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification and also plays a role in cartilage mineralization
• Collagen Type X is elevated in many tumor types with expression being localized in the vasculature
• Mutations to the gene COL10A1, which encodes human Collagen Type X, is associated with Schmid type metaphyseal chondrodysplasia
• Applications Reported: This X53 antibody has been reported for use in microscopy, immunohistochemical staining, ELISA, and immunocytochemistry
• Applications Tested: This X53 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.