7105450

CD63 Antibody (SPM524), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD63

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse

Gene Accession No.

P08962

Gene ID (Entrez)

967

Immunogen

Recombinant human CD63 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Clone

SPM524

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Autophagy, Cytokine Research

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin

Gene Symbols

CD63

Isotype

IgG1 κ

Purification Method

Protein A purified

Test Specificity

This product is specific for CD63

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Description

  • CD63 Monoclonal specifically detects CD63 in Human, Mouse samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Antigen:
CD63

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P08962

Gene ID (Entrez):
967

Immunogen:
Recombinant human CD63 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
SPM524

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Autophagy, Cytokine Research

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin

Gene Symbols:
CD63

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This product is specific for CD63

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Antigen:
TfR (Transferrin R)

Dilution:
Western Blot 0.25-0.5ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P02786

Gene ID (Entrez):
7037

Immunogen:
KG1 acute myeloid leukemia cell line

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
DF1513

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cell Biology, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CD71 antigen, CD71TRFR, p90, T9, TfR, TFR1, TR, transferrin receptor (p90, CD71), transferrin receptor protein 1, Trfr

Gene Symbols:
TFRC

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
It recognizes a ∼90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

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Antigen:
CD74

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Frozen 0.5-1ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P044233

Gene ID (Entrez):
972

Immunogen:
B lymphoblastoid cell line HFB1

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
BU45

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Immunology

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated), CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAGgamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLADG, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen-associated invariant chain, Ia-associated invariant chain, Ia-GAMMA, Ii, MHC HLA-DR gamma chain, p33

Gene Symbols:
CD74

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74. Its epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

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Antigen:
CD79A

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P11912

Gene ID (Entrez):
973

Immunogen:
A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
JCB117

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Immunology, Phospho Specific

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CD79a antigen, CD79A antigen (immunoglobulin-associated alpha), CD79a molecule, immunoglobulin-associated alpha, IGAB-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB1, MB-1, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Gene Symbols:
CD79A

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as wel