7105629

Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Enolase 2/Neuron-specific Enolase

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page, Immunofluorescence 1-2ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat

Gene Alias

2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE

Gene Symbols

ENO2

Isotype

IgG

Purification Method

Protein A or G purified

Test Specificity

Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Clone

ENO2/1375

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez)

2026

Immunogen

A synthetic peptide corresponding to aa416-433 of human NSE gamma.

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

50 kDa

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Description

  • Enolase 2/Neuron-specific Enolase Monoclonal specifically detects Enolase 2/Neuron-specific Enolase in Human, Mouse, Rat samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular).

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Fischer Scientific

7105629

Mouse Monoclonal Antibody...


Antigen:
Enolase 2/Neuron-specific Enolase

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page, Immunofluorescence 1-2ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat

Gene Alias:
2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE

Gene Symbols:
ENO2

Isotype:
IgG

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Clone:
ENO2/1375

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
2026

Immunogen:
A synthetic peptide corresponding to aa416-433 of human NSE gamma.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
50 kDa

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7105639

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Antigen:
ASRGL1

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, SDS-Page, Immunofluorescence 0.5-1ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
ALP1, ALPL-asparagine amidohydrolase, asparaginase like 1, asparaginase-like 1 protein, Asparaginase-like protein 1, CRASH, EC 3.5.1.1, FLJ22316, L-asparaginase

Gene Symbols:
ASRGL1

Isotype:
IgG

Purification Method:
Protein A or G purified

Test Specificity:
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

Clone:
CRASH/1289

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
__

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
80150

Immunogen:
Recombinant human ASRGL1 fragment

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__

Img

Fischer Scientific

7105639

Mouse Monoclonal Antibody...


Antigen:
ASRGL1

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, SDS-Page, Immunofluorescence 0.5-1ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
ALP1, ALPL-asparagine amidohydrolase, asparaginase like 1, asparaginase-like 1 protein, Asparaginase-like protein 1, CRASH, EC 3.5.1.1, FLJ22316, L-asparaginase

Gene Symbols:
ASRGL1

Isotype:
IgG

Purification Method:
Protein A or G purified

Test Specificity:
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

Clone:
CRASH/1289

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
__

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
80150

Immunogen:
Recombinant human ASRGL1 fragment

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__

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Antigen:
Adiponectin/Acrp30

Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page, Immunofluorescence 1-2ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
ACDC, ACRP30ADPN, adipocyte, C1Q and collagen domain containing, adiponectin, adiponectin, C1Q and collagen domain containing, AdipoQ, ADIPQTL1, apM-1, APM1APM-1, C1q and collagen domain-containing protein, GBP28apM1, Gelatin-binding protein

Gene Symbols:
ADIPOQ

Isotype:
IgG2b

Purification Method:
Protein A or G purified

Test Specificity:
This MAb reacts with adiponectin, an adipocytokine. Adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.

Clone:
ADPN/1370

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Diabetes Research, Lipid and Metabolism

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
9370

Immunogen:
Recombinant human Adiponectin protein fragment

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__