Glucocorticoid receptor (NR3C1) Monoclonal Antibody (BuGR2), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This BuGR2 monoclonal antibody reacts with mouse glucocorticoid receptor NR3CR1
• This clone has been also reported to cross-react with human NR3CR1
• This BuGR2 antibody is recommended to be used with the Foxp3/Transcription factor staining buffer set (Product # 00-5523-00)
• Applications Reported: This BuGR2 antibody has been reported for use in flow cytometric analysis
• This clone has been also reported to cross-react with Human, Non-human primate, Rabbit, Rat, Sheep, Xenopus, Yeast NR3CR1
• Applications Tested: This BuGR2 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol
• Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at www.thermofisher.com/flowprotocols
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors
• Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins
• The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus
• Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart
• Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined
• Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance
• Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation
• GR binds to known steroids such as dexamethasone with nanomolar affinity