7162956
Ketohexokinase Isoform C, Mouse anti-Human, Clone: 5F1-3F5, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Ketohexokinase Isoform C
Dilution
Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
Gene Symbols
KHK
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
Clone
5F1-3F5
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Signaling
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_000212
Immunogen
A full-length human recombinant Ketohexokinase, isoform C.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat
- No
- MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting
- Ketohexokinase (UniProt: P50053; also known as EC: 2.7.1.3, Hepatic fructokinase) is encoded by the KHK gene (Gene ID: 3795) in human
- Ketohexokinase is a homodimeric cytosolic enzyme in that catalyzes the conversion of fructose to fructose 1 phosphate
- It is most abundant in the liver, where it may constitute up to 0.6% of the total liver protein
- It has also been reported in kidney, spleen, gut, and pancreas
- Very low levels are also found in adrenal, muscle, brain, and eyes
- It has multiple fructose binding sites
- There are two major isoforms of this enzyme ketofructokinase C and ketofructokinase A
- Due to its high Km (∽ 28 mM), fructose is preferentially metabolized by the C isoform
- Ketohexokinase -C is unique among sugar kinases in that its metabolism of fructose is associated with a rapid depletion of intracellular ATP
- Unlike glucokinase, in which excessive phosphorylation of glucose is prevented by a negative feedback system, the metabolism of fructose by ketohexokinase-C results in rapid phosphorylation with a fall in intracellular phosphate and ATP
- If the ATP levels fall significantly then the protein synthesis is transiently stopped and cells may develop features that are consistent with ischemia
- Mutations in KHK gene has been linked to fructosuria, a clinically benign condition that is characterized nu the incomplete metabolism of fructose in the liver, leading to its urinary excretion
- (Ref.: Diggle, CP et al., (2009)
- J
- Histochem
- Cytochem
- 57(8): 763-774; Hayward, BE., and Bonthron, DT (1998)
- Eur
- J
- Biochem
- 257(1); 85-91).
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Antigen: Ketohexokinase Isoform C
Dilution: Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
Gene Symbols: KHK
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
Clone: 5F1-3F5
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000212
Immunogen: A full-length human recombinant Ketohexokinase, isoform C.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Ketohexokinase Isoform C
Dilution:
Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
Gene Symbols:
KHK
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
Clone:
5F1-3F5
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000212
Immunogen:
A full-length human recombinant Ketohexokinase, isoform C.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: carboxypeptidase M
Dilution: Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat
Gene Alias: CPM;CP-M
Gene Symbols: Cpm
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Clone: 7F9
Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001101568.1
Immunogen: Membrane fraction of E18.5 embryonic rat lung.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
carboxypeptidase M
Dilution:
Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat
Gene Alias:
CPM;CP-M
Gene Symbols:
Cpm
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Clone:
7F9
Applications:
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001101568.1
Immunogen:
Membrane fraction of E18.5 embryonic rat lung.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: PEG
Dilution: Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Polyethylene Glycol
Gene Symbols: __
Isotype: IgM κ
Purification Method: Purified by ion-exchange chromatography
Test Specificity: Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Clone: AGP4
Applications: ELISA, Immunohistochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Gene ID (Entrez): __
Immunogen: Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PEG
Dilution:
Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Polyethylene Glycol
Gene Symbols:
__
Isotype:
IgM κ
Purification Method:
Purified by ion-exchange chromatography
Test Specificity:
Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Clone:
AGP4
Applications:
ELISA, Immunohistochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: PEG
Dilution: ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Polyethylene Glycol
Gene Symbols: __
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone: 6.3
Applications: ELISA
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Gene ID (Entrez): __
Immunogen: PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PEG
Dilution:
ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Polyethylene Glycol
Gene Symbols:
__
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone:
6.3
Applications:
ELISA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Gene ID (Entrez):
__
Immunogen:
PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.