7162957
carboxypeptidase M, Mouse anti-Rat, Clone: 7F9, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
carboxypeptidase M
Dilution
Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Rat
Gene Alias
CPM;CP-M
Gene Symbols
Cpm
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Clone
7F9
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Signaling
Formulation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001101568.1
Immunogen
Membrane fraction of E18.5 embryonic rat lung.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Related Products
Description
- Anti-carboxypeptidase M, clone 7F9, Cat
- No
- MABS1925, is a mouse monoclonal antibody that detects Carboxypeptidase M and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting
- Carboxypeptidase M (UniProt: D4A9Q5; also known as CPM) is encoded by the Cpm gene (Gene ID: 314855) in rat
- Rat CPM a glycosylphosphatidylinositol (GPI) anchored metallo-carboxypeptidase that cleaves basic carboxyl-terminal amino acids from various peptides at neutral pH
- Rat CPM is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form
- The mature CPM displays high specificity and cleaves only C-terminal arginine or lysine residues in a variety of peptide substrates
- It is shown to be expressed in the lung and placenta at high levels
- Its expression is detected in alveolar cells lacking T1alpha, a type I cell marker protein found in adult lung
- Carboxypeptidase M also serves as a macrophage differentiation marker, which is preferentially induced in epithelioid cells of all granuloma types studied, but not in resting macrophages
- Hence, it can be employed for detection of minute granuloma even in the presence of non-granulomatous macrophages
- Clone 7F9 recognizes CPM expressed in late embryonic rat lung to postnatal day 1
- (Ref.: Fujiwara, N., et al
- (2007), Respirology 12(1); 54-62; Tsakiris, I., et al
- (2012)
- Lab
- Invest
- 92(3); 345-361).
Compare Similar Items
Show Difference
Antigen: carboxypeptidase M
Dilution: Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Rat
Gene Alias: CPM;CP-M
Gene Symbols: Cpm
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Clone: 7F9
Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001101568.1
Immunogen: Membrane fraction of E18.5 embryonic rat lung.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
carboxypeptidase M
Dilution:
Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Rat
Gene Alias:
CPM;CP-M
Gene Symbols:
Cpm
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
Clone:
7F9
Applications:
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001101568.1
Immunogen:
Membrane fraction of E18.5 embryonic rat lung.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: PEG
Dilution: Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Polyethylene Glycol
Gene Symbols: __
Isotype: IgM κ
Purification Method: Purified by ion-exchange chromatography
Test Specificity: Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Clone: AGP4
Applications: ELISA, Immunohistochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Gene ID (Entrez): __
Immunogen: Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PEG
Dilution:
Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Polyethylene Glycol
Gene Symbols:
__
Isotype:
IgM κ
Purification Method:
Purified by ion-exchange chromatography
Test Specificity:
Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Clone:
AGP4
Applications:
ELISA, Immunohistochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: PEG
Dilution: ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Polyethylene Glycol
Gene Symbols: __
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone: 6.3
Applications: ELISA
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Gene ID (Entrez): __
Immunogen: PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PEG
Dilution:
ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Polyethylene Glycol
Gene Symbols:
__
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone:
6.3
Applications:
ELISA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Gene ID (Entrez):
__
Immunogen:
PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ST8SIA1
Dilution: Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Alpha-N-acetylneuraminide alpha-2;8-sialyltransferase;EC: 2.4.99.8;Alpha-2;8-sialyltransferase 8A;Ganglioside GD3 synthase;Ganglioside GT3 synthase;Sialyltransferase 8A;SIAT8-A;Sialyltransferase St8Sia I;ST8SiaI
Gene Symbols: ST8SIA1;SIAT8;SIAT8A
Isotype: IgM κ
Purification Method: Purified by ion-exchange chromatography
Test Specificity: Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
Clone: HIC0-3C5
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_003025.1
Immunogen: Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ST8SIA1
Dilution:
Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Alpha-N-acetylneuraminide alpha-2;8-sialyltransferase;EC: 2.4.99.8;Alpha-2;8-sialyltransferase 8A;Ganglioside GD3 synthase;Ganglioside GT3 synthase;Sialyltransferase 8A;SIAT8-A;Sialyltransferase St8Sia I;ST8SiaI
Gene Symbols:
ST8SIA1;SIAT8;SIAT8A
Isotype:
IgM κ
Purification Method:
Purified by ion-exchange chromatography
Test Specificity:
Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
Clone:
HIC0-3C5
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_003025.1
Immunogen:
Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.