beta 1 Adrenergic Receptor Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity purified antibody is directed against human beta 1 adrenergic
• A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, and rhesus macaque
• Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine
• Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family
• Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3)
• ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence
• BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism
• In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function
• Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system
• Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.