CD56 (NCAM) Monoclonal Antibody (5tukon56), eBioscience™, Invitrogen™

Description

• Description: This 5tuckon56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM)
• CD56 is a highly glycosylated transmembrane molecule expressed in both the developing and adult nervous system and plays a role in neurogenesis, migration, neurite extension, and the homotypic adhesion of neural cells
• In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells
• For flow cytometric analysis of human CD56, we recommend clones CMSSB or TULY56
• Applications Reported: This 5tukon56 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy
• Applications Tested: This 5tukon56 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue sections using low or high pH antigen retreival and can be used at less than or equal to 1 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa
• CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity
• Polysialic modification results in reduction of CD56-mediated cell adhesion
• Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins
• CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma
• Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors
• Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.