ABS150825UG
SREBP-1, Rabbit anti-Human, Polyclonal, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABS150825UG-Each-of-1 | In Stock | ₹ 16,456.61 |
ABS150825UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
SREBP-1
Classification
Polyclonal
Dilution
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SREBP-1 in human adrenal gland and human kidney tissues.
Gene Alias
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species
Rabbit
Molecular Weight of Antigen
∽120 kDa observed; 121.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μg
Research Discipline
Signaling
Test Specificity
This rabbit polyclonal antibody detects Sterol regulatory element-binding protein 1 in human.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
SREBF1;BHLHD1;SREBP1
Immunogen
KLH-conjugated linenar peptide corresponding to 14 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1. The Immunogen sequence is conserved in SREBP-1A, B, and C and in isoform 4.
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Isotype
IgG
Related Products
Description
- Anti-SREBP-1, Cat
- No
- ABS1508, is a rabbit polyclonal antibdody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Sterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human
- SREBP-1 is a multi-pass membrane protein that acts as at transcriptional activator required for lipid homeostasis
- Six isoforms of SREBP-1 have been described that are produced by alternative splicing
- SREBP-1 is expressed in a wide variety of tissues and is most abundant in liver and adrenal gland
- In fetal lung and liver it is expressed in high levels
- SREBP-1A predominates in hepatoma cell lines while isoform SREBP-1C is predominantly found in liver, adrenal gland, and ovary
- Both isoforms IA and IC is found in kidney, brain, white fat, and muscle
- SREBP-1 regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway
- It binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') and has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3')
- SREBP-1 has two cytoplasmic domains (aa 1-487 and 567-1147; two helical domains (aa 488-508 and 548-568), and a luminal domain (aa 509-547)
- In the absence of sterols, it moves from the endoplasmic reticulum to the Golgi
- At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER
- In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease
- The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane.
Compare Similar Items
Show Difference
Antigen: SREBP-1
Classification: Polyclonal
Dilution: Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SREBP-1 in human adrenal gland and human kidney tissues.
Gene Alias: Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species: Rabbit
Molecular Weight of Antigen: ∽120 kDa observed; 121.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μg
Research Discipline: Signaling
Test Specificity: This rabbit polyclonal antibody detects Sterol regulatory element-binding protein 1 in human.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: SREBF1;BHLHD1;SREBP1
Immunogen: KLH-conjugated linenar peptide corresponding to 14 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1. The Immunogen sequence is conserved in SREBP-1A, B, and C and in isoform 4.
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Isotype: IgG
Antigen:
SREBP-1
Classification:
Polyclonal
Dilution:
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SREBP-1 in human adrenal gland and human kidney tissues.
Gene Alias:
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species:
Rabbit
Molecular Weight of Antigen:
∽120 kDa observed; 121.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity:
25 μg
Research Discipline:
Signaling
Test Specificity:
This rabbit polyclonal antibody detects Sterol regulatory element-binding protein 1 in human.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
SREBF1;BHLHD1;SREBP1
Immunogen:
KLH-conjugated linenar peptide corresponding to 14 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1. The Immunogen sequence is conserved in SREBP-1A, B, and C and in isoform 4.
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Isotype:
IgG
Antigen: phospho-Neph1 (Tyr637/638)
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Signaling
Test Specificity: __
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Applications: Western Blot
Conjugate: __
Formulation: Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, 0.02% Sodium Azide and 30% glycerol.
Gene Symbols: Kirrel; Kirrel1; Neph1
Immunogen: Linear peptide corresponding to the cytoplasmic domain of mouse phospho-Neph1 (Tyr637/638).
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Isotype: __
Antigen:
phospho-Neph1 (Tyr637/638)
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μL
Research Discipline:
Signaling
Test Specificity:
__
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Applications:
Western Blot
Conjugate:
__
Formulation:
Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, 0.02% Sodium Azide and 30% glycerol.
Gene Symbols:
Kirrel; Kirrel1; Neph1
Immunogen:
Linear peptide corresponding to the cytoplasmic domain of mouse phospho-Neph1 (Tyr637/638).
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Isotype:
__
Antigen: Neph1
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Signaling
Test Specificity: __
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Applications: Immunoassay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: __
Formulation: Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, 0.02% Sodium Azide and 30% glycerol.
Gene Symbols: Kirrel; Kirrel1; Neph1
Immunogen: His-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Neph1.
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Isotype: __
Antigen:
Neph1
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μL
Research Discipline:
Signaling
Test Specificity:
__
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Applications:
Immunoassay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
__
Formulation:
Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, 0.02% Sodium Azide and 30% glycerol.
Gene Symbols:
Kirrel; Kirrel1; Neph1
Immunogen:
His-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Neph1.
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Isotype:
__
Antigen: phospho-Ubiquitin (Ser65)
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Signaling
Test Specificity: Target specificity was verified using the unconjugated antibody (ABS1513-I). Only Ser65-phosphorylated ubiquitin (Ub) and ubiquitinated proteins (including oligoUb and polyUb) containing Ser65-phosphorylated ubiquitin were detected. This antibody exhibits no reactivity toward Ub or ubiquitinated proteins in the absence of Ub Ser65 phosphorylation.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Applications: Immunocytochemistry
Conjugate: Alexa Fluor 488
Formulation: Purified rabbit polyclonal antibody in PBS with 1.5% BSA and 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: KLH-conjugated linear peptide corresponding to the ubiquitin target region sequence containing phosphorylated Ser65.
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Isotype: __
Antigen:
phospho-Ubiquitin (Ser65)
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μL
Research Discipline:
Signaling
Test Specificity:
Target specificity was verified using the unconjugated antibody (ABS1513-I). Only Ser65-phosphorylated ubiquitin (Ub) and ubiquitinated proteins (including oligoUb and polyUb) containing Ser65-phosphorylated ubiquitin were detected. This antibody exhibits no reactivity toward Ub or ubiquitinated proteins in the absence of Ub Ser65 phosphorylation.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Applications:
Immunocytochemistry
Conjugate:
Alexa Fluor 488
Formulation:
Purified rabbit polyclonal antibody in PBS with 1.5% BSA and 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
KLH-conjugated linear peptide corresponding to the ubiquitin target region sequence containing phosphorylated Ser65.
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Isotype:
__