MilliporeSigma™ Rabbit anti-Telo-Collagen Type I, A1/COL1A1, Polyclonal,

Description

• Specifically detects Telo-Collagen Type I, A1/COL1A1 in Human, Mouse, Sheep samples, and it is validated for Flow Cytometry, Immunohistochemistry, Western Blotting Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human
• Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones
• Type I collagen consists of two alpha I chains and one alpha 2 chain
• Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a
• 1-22) and two propeptide sequences (a.a
• 23-161 and a.a
• 1219 –1464), the removal of which yields the mature alpha-1(I) chain
• The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a
• 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a
• 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a
• 1193-1218; numbering based on the prepro-form)
• Collagen can be extracted from tissue via either enzymatic or non-enzymatic means
• Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin
• On the other hand, collagen preparations obtained with non-enzymatic means (e.g
• by acid extraction) have the intact telopeptides at both ends.